Real-Time PCR

DNA amplification

One of our newest peices of equipment is the QuantStudio™ 12K Flex Real-Time PCR System, offering maximum throughput, flexibility and scalability.

This machine can simultaneously run up to four 3072 reaction QuantStudio 12K Flex OpenArray Plates in around 4 hours. At the CSCR we have 4 thermal cycling blocks: OpenArray, TaqMan Array Card, 384 well and a Fast 96-well blocks. Block changes are really quick and simple to do. The different blocks allow you to analyze up to 12,000 data points from a single run. This machine uses a white light LED optics system instead of the laser that is used in the 7900HT machine.

OpenArray: A nanolitre fluidics platform for low volume, solution-phase reactions.

The QuantStudio 12K Flex OpenArray plates are microscope slide-sized and are arranged in 48 subarrays of 64 through holes, with a total of 3072 through-holes for individual 33nl reactions. Hydrophilic and hydrophobic compounds are used to retain reagents in the through-holes via surface tension.

You can find more details of the qPCR machine's capabilities in the brochure: Download qPCR Brochure or at the manufacturer's website.


The qPCR machine is run by Mrs Donna Leaford. It is maintained and run by the Hendrich lab on behalf of SCI

For ruther information regarding the use of the qPCR machine, please contact

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