Nuclear reprogramming of tissue cells back to an embryonic cell state is generally inefficient

Histone variant macroH2A marks embryonic differentiation in vivo and acts as an epigenetic barrier to induced pluripotency.

Nuclear reprogramming of tissue cells back to an embryonic cell state is generally inefficient. This reflects the stability of tissue cells. A long lasting question in biology concerns what particular mechanisms acquired during embryonic development are responsible for this stability. Our study identifies for the first time a link between a mechanism, macroH2A, and embryonic development, which antagonises reprogramming to an embryonic cell state.

Authors: Vincent Pasque, Aliaksandra Radzisheuskaya, Astrid Gillich, Richard P. Halley-Stott, Maryna Panamarova, Magdalena Zernicka-Goetz, M. Azim Surani, José C.R. Silva

Publication details: http://jcs.biologists.org/content/early/2012/10/12/jcs.113019.full.pdf+html

E6.5 female X-GFP mouse conceptus wholemount immunofluorescence against macroH2A.1 (red in merge pan

¬E6.5 female X-GFP mouse conceptus wholemount immunofluorescence against macroH2A.1 (red in merge panel) and GFP (green in merge panel). MacroH2A.1 is highly expressed in the visceral endoderm (VE) and to some extent in the extra embryonic ectoderm (TE) but is not detected in the epiblast (EPI), precursor of all somatic lineages (mosaic X-GFP expression due to random X chromosome inactivation). DAPI is in blue. Images are projected confocal Z-sections. Scale bar = 20 µm. (Image credit: Vincent Pasque and Astrid Gillich.)

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