Chromatin decondensation and nuclear softening accompany Nanog downregulation in ESCs
We used biophysical techniques to study nuclear mechanics and higher-order chromatin organisation in embryonic stem cells. We investigated two states: a naïve and pre-committed state (i.e. the state that immediately precedes commitment of the embryonic stem cells). We found that cells in a pre-committed state have a significantly softer nucleus and more globally decondensed chromatin. We hypothesise that this combination of physical factors relates to the plasticity of the pre-committed stem cell in responding to chemical and physical cues from its environment.
Biophys J. 2012 Nov 21;103(10):2060-70.