MBD3/NuRD Facilitates Induction of Pluripotency

Induced pluripotency is a recently discovered way of making cells equivalent to those present in the embryo and with the ability to form all the tissues in the body from adult cells. It involves introducing genes that are normally present in pluripotent cells into adult cells. This breakthrough discovery has opened unprecedented new perspectives in both regenerative medicine and drug discovery. However, before this technology can be used in a clinical setting, it is important to understand what are the mechanisms surrounding the generation of these induced pluripotent stem cells (iPSCs). This research will lead to the creation of even better and safer iPSCs bringing the applications in regenerative medicine a step closer. In our study we assessed the role of a multi-protein DNA regulator termed NuRD during the generation of induced pluripotent stem cells (iPSCs) from skin and brain cells. Our study describes for the first time that the key DNA regulator NuRD is required for the efficient generation of iPSCs. In addition, its enhanced activity can further boost the generation of iPSCs. This demonstrates that NuRD, an essential DNA regulator for the generation of specialised cells such as skin and brain cells, is also key to the reversion of the specialised cells back into an early embryonic state (iPSCs).

Full article in Cell Stem Cell

Publication details:

Santos RL, Tosti L, Radzisheuskaya A, Caballero IM, Kaji K, Hendrich B, Silva JCR. MBD3/NuRD Facilitates Induction of Pluripotency in a Context-Dependent Manner. Cell Stem Cell. DOI: http://dx.doi.org/10.1016/j.stem.2014.04.019

Reprogramming of skin cells

Reprogramming of skin cells boosted by NuRD activity. Green fluorescence indicates emerging iPS cell colonies.

Image credit: Rodrigo L. dos Santos

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