SCI scientists reset human stem cells to earliest developmental state

SCI researchers have managed to induce a ground state by rewiring the genetic circuitry in human embryonic and induced pluripotent stem cells. Their “reset” cells share many of the characteristics of authentic naïve embryonic stem cells isolated from mice, suggesting that they represent the earliest stage of development.

The study, published in Cell, will lead to a better understanding of human development and could in future allow the production of safe and more reproducible starting materials for a wide range of applications including cell therapies.

Read more: and

Read the published article in Cell

Publication details: Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A. Resetting transcription factor controle circuitry towards ground state pluripotency in human. Cell Volume 158, Issue 6, p1254–69, 11 September 2014.

Human reset cells-2

The left panel shows the morphology of human reset cells. The dome-shaped colony of human reset cells is similar to mouse naïve cells. EOS-GFP, which carries Oct4 distal enhancer, is thought to be expressed in mouse naïve cells. Human reset cells express EOS-GFP (right panel), whereas human conventional pluripotent stem cells do not express EOS-GFP.

Image credit: Yasuhiro Takashima


Web design by Studio 24 / Back to top