Selection and dynamics of embryonic stem cell integration into early mouse embryos

The Nichols lab used live imaging to investigate how donor embryonic stem cells are selected for integration into host embryos during the formation of murine preimplantation chimaeras. We found that cells in the process of differentiation are acutely eliminated soon after injection and that there is a second wave of cell death of the donated cells coinciding with the second lineage segregation of the host embryo. Some of the differentiating injected cells apparently revert to the undifferentiated state during integration, implying a hitherto unsuspected capacity of the early mouse embryo for ‘reprogramming’. Read more in Development.

Stoyana A, Kalkan T, Humphreys P, Riddell A, Scognamiglio, Trumpp A, Nichols J. Selection and dynamics of embryonic stem cell integration into early mouse embryos. Development. PMID: 26586221

Mouse chimaera

Mouse chimaera showing injected embryonic stem cells (red) undergoing cell death by apoptosis. Image Credit: Stoyana Alexandrova and Peter Humphreys

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