Dr Matthias Zilbauer
Intestinal stem cell biology
Departmental Affiliation: Department of Paediatrics
The Zilbauer group's main research interest lies in the field of mucosal immunology of the GI tract during health and paediatric diseases particularly Inflammatory Bowel Diseases (IBD) and Necrotising Enterocolitis (NEC). More specifically, the group has been investigating the role of epigenetic mechanisms (i.e. DNA methylation) in regulating gene expression and cellular function of the human intestinal epithelium.
In close collaboration with the Koo group, human intestinal epithelial organoids are being developed as functional models to investigate intestinal epithelial biology during health and disease.
In addition to organoids derived from intestinal mucosal samples, induced pluripotent stem cells (iPSCs) can be differentiated into intestinal epithelium, which is the subject of an ongoing collaboration with the Vallier group.
Our overall aim is to further develop and validate these highly promising organoid culture models into powerful and reliable tools, which can then be used to develop novel treatments as well as testing existing drugs and hence allowing a personalized treatment approach.
Intestinal epithelial organoid cultures derived from mucosal samples obtained from 3 gut segments: Ascending Colon, Sigmoid Colon, Terminal Ileum. Images were taken at Day 1 and Day 6 post seeding of isolated intestinal crypts into matrigel.