A Kurowski

Agata Kurowski

2010 - 2015


Studentship Details

 

Dates of Study: Oct 2010 - Mar 2015

Studentship Sponsor: Medical Research Council

PhD Supervisor: Dr Jennifer Nichols

Thesis Title: The establishment and maintenance of the pluripotency network in the pre-implantation mouse embryo

Public Outreach: Agata volunteered on the Stem Cell Institute stand at the University of Cambridge annual Science Festival in 2014 and 2015. Agata also supervised a student on the Nuffield Programme in 2015 and a Biochemistry undergraduate student in 2010.

 

Post PhD

After her PhD, Agata took up a PostDoc position in the lab of Dr Jennifer Nichols at the Cambridge Stem Cell Institute, UK.

In February 2016, Agata began working as a PostDoc in Philippe Soriano's lab at Mount Sinai Hospital, New York, USA.

Image 1

A) Maximal projection of a representative confocal image of an Oct4 het blastocyst stained for β-Catenin and Oct4 with nuclear DAPI.

B) Maximal projection of a representative confocal image of a maternal-zygotic Oct4-mutant blastocyst stained for β-Catenin and Oct4 with nuclear DAPI.

Plain English

The molecular circuitry controlling embryonic stem cell (ESC) self-renewal in vitro has been the subject of many studies but the essential question of how it is engineered in the founder tissue of the embryo has not been investigated. To determine the transcriptional regulation of establishment and maintenance of pluripotency in the emerging mouse epiblast, embryos carrying lethal mutations in the key pluripotency factors Oct4 and Nanog were studied using quantitative immunofluorescence (QIF) with confocal microscopy.

Key Publications

  1. Le Bin GC et al. (2014). Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. Development. PMID: 24504341.
  2. Blanco S et al. (2011). The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells to differentiatePLoS Genet. PMID: 22144916.
  3. Arthold S et al. (2011). Mechanistic insights into chromosome-wide silencing in X inactivation. Hum Genet. PMID: 21567178.

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