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Genomics

Preparation of DNA/RNA libraries for Next Generation Sequencing projects

Next Generation Sequencing has become indispensable for most research projects. The Cambridge Stem Cell Institute Genomics Facility offers support to all research groups to produce high quality libraries as well as a reliable and fast turnaround for standard library preparation. We have experience in a wide variety of different library preparation techniques, and we are always happy to try out new things as well. Our services are available to both internal and external users from the academic and private sectors.

Services

  • Project planning and advice about your NGS project
  • Covaris E220 shearing of DNA/RNA samples; chromatin samples
  • Sample QC
  • Library preparation and QC
  • Advice on choice of sequencing platform
  • Close interaction with Bioinformatics support facility

 

Range of sequencing applications

  • Transcriptome/mRNA sequencing
  • Chip-seq
  • Small RNA sequencing
  • Single cell transcriptome sequencing
  • Whole Genome Bisulfite Sequencing
  • Reduced Representation Bisulfite Sequencing
  • Genomic resequencing libraries
  • ATAC sequencing
  • Specialist techniques (tRNA, riboseq, Chirp, MNase, RNA bisulfite seq, CRISPR, transposon mutagenesis screens, ultra low input samples, …)
  • Individual projects and non-established methods will always be considered!

 

Sequencing

The sequencing itself is not done in-house, but we have a collaboration with the CIGC here in Cambridge which allows us access to the latest Illumina sequencing technology. Currently we can offer a wide range of throughput needs on the HiSeq4000 and the Novaseq.

If you are using the NGS library facility, we will submit your libraries directly to the genome core at CIGC. If you are making your own libraries and would like access to the sequencing core, your group will need to set up an account with CIGC, and submit the SLX submission files for approval to Maike Paramor (mp600@cam.ac.uk).

 

Data Analysis

The Institute has a bioinformatics facility, which will provide support with any aspect of your data analysis. Please contact Dr Irina Mohorianu (iim22@cam.ac.uk)

 

Contact

The Gene Service Facility is managed by .

 

How to book

For consultation about your experimental design, please contact Maike Paramor

Information about sample submission can be found here.

Please find here the online sample submission form, which needs to be filled in at the time of your sample submission.

Help on the sample submission form can be found here.

This is a list of FAQs

 

To make an equipment booking, please go to the online Booking System.