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Warning Text %XTableStyleMedium2PivotStyleLight16`MinternalʕSheet3,  ;W8_WTCSCRTennis Court Road Cambridge 01223 760293 PO Number DateName Tel numbere-mail Group leader InstituteBuilding Active WT MEFActive DS red MEFActive DR4 MEFInactivated DS red MEF500ug550ul250ug100ulSerumES serum In house N25mlBatch tested B2710mlBatch tested Neural basal500ml Liquid broth20ml Addenbrooks BabrahamSangerprice Number of vialsCost ES serum 50mlSizeFeedersN2B27 MycoplasmabFGF2 Activin A Actavin A-2BMP2 TAT-CreBMP4 Ms LIF Hu LIF Ms FGF4 TAT-Dre2mg50 ul at 0.5 mM1mg400ug100ul at 0.2mMStock tested GF GF not batch testedAliquoted reagents50ul80ul10ul110ulInactivated DR4 MEFActavin A-2 500ug/mlbFGF2 50ug/mlMs LIF 10ug/ml Delivery Taxi Postcode InhibitorsChiron60ul45ul30ul15ulPD0320ul5ulSCI Tissue Culture Order Form PO amount Chiron 10mM PD03 10mMDelivery addressGroupHu LIF 10ug/ml Actavin A20.5mg/ml4ml 10mM HCL per 250ug vial Activin A500ul 10mM HCL per 250ug vialFGF2 50ug/ml 1vial into 10ml PBS Murine LIF10ug/ml Human LIF1g of Chiron in 214.9 ml DMSO1g PDO3 in 207.8 ml DMSO10mM"1mg vial to 100ml PBS with 0.1%BSARoutineReagent preparation Tissue-culture@cscr.cam.ac.uk500ulInactive WT MEF tPlease note N2B27 testing takes several weeks, these items should be ordered months in advance to guarentee a supplyActive GFP MEFConditioned media Inactivated DR4 MEF HomoLimited supply Grand Total!Non SCI must have a MTA in place 7Finance: Please recharge Growth Factor supply onto PSGF2Finance: Please recharge aliqouts supply onto PSMH~2-4x1068-9x1066Inactivated DR4 MEF Het - reduced puromycin resistancePlease note condition media production takes a couple of months. 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