The healthy cells immediately surrounding a tumour become more stem cell-like and support cancer growth, reveals a new study published in Nature. The discovery was made during a research collaboration between Dr Joo-Hyeon Lee at the Wellcome – MRC Cambridge Stem Cell Institute and Dr Ilaria Malanchi at the Francis Crick Institute.
The researchers developed state-of-the-art techniques to study the tissue around a tumour – called the tumour microenvironment – known to influence the growth and spread of cancer, as well as treatment response.
“Our new technique allows us to study changes to cells in the tumour microenvironment with unprecedented precision,” says Dr Ilaria Malanchi, who joint-led the project. "This helps us to understand how these changes relate to tumour growth and metastasis, allowing us to develop better strategies to treat the disease.
“We discovered that non-cancerous cells in the tumour microenvironment regress back into a stem-cell like state, and actually support cancer growth. By corrupting its neighbours, cancer transforms its local environment to support its own survival.”
Colourful cancer marker
This new technique relies on cancer cells engineered to release a cell-penetrating fluorescent protein that gets taken up by its neighbouring cells. These colour-labelled cells can be identified and compared to other (unlabelled) cells that have not come into contact with the tumour. The researchers used this approach in mice to study the cells around breast cancer that had spread to the lungs. Data from Dr Alessandro Ori's lab at the Fritz Lipmann Institute in Germany confirmed that the labelled cells produced different proteins to unlabelled cells.
The researchers found labelled cells from the lung to have stem cell-like features, unlike the lung cells found outside of the tumour microenvironment. The team showed that those cells from the mouse lungs supported tumour growth when mixed with tumour cells in 3D culture in the lab, suggesting that they help the cancer to survive and grow.
Stem cells to mini lungs
In order to further test the potential of the stem-cell like cells in the tumour microenvironment, Dr Joo-Hyeon Lee used the engineered cells to grow lung organoids, or ‘mini-lungs’.
The unlabelled healthy lung cells formed mini-lungs, mostly made up of alveolar epithelial cells which line the lung’s alveoli – the tiny sacs where gas exchange takes place. By comparison, the labelled cells taken from the tumour microenvironment unexpectedly formed mini-lungs with a wider range of cell types.
“To our amazement, we found that cells receiving proteins from adjacent cancer cells obtained stem-cell-like features”, explains Dr Joo-Hyeon Lee, joint senior author of the paper. “They could change their fate to become different cell types. It demonstrates the powerful influence that cancer exerts over its neighbouring cells, making them liable to change easily.”
The researchers hope that their approach will be used by other scientists looking to gain a deeper understanding of the local changes triggered by cancer which help it to survive, spread and develop resistance to treatments.
The potential applications are not confined to cancer – a similar approach could enable scientists to study interactions between different cell types in the body.
Publication details:
Metastatic-niche labelling reveals parenchymal cells with stem features.M L Ombrato, E Nolan, I Kurelac, A Mavousian, V Bridgeman, I Heinze,
P Chakravarty, S Horswell, E Gonzalez-Gualda, G Matacchione, A Weston, J Kirkpatrick, E Husain, V Speirs, L Collinson, Al Ori, J-H Lee* & I Malanchi* is published in Nature. https://www.nature.com/articles/s41586-019-1487-6