Preparation of DNA/RNA libraries for Next Generation Sequencing projects
Next Generation Sequencing has become indispensable for most research projects. The Cambridge Stem Cell Institute Genomics Facility offers support to all research groups to produce high quality libraries as well as a reliable and fast turnaround for standard library preparation. We have experience in a wide variety of different library preparation techniques, and we are always happy to try out new things as well. Our services are available to both internal and external users from the academic and private sectors.
Services
- Project planning and advice about your NGS project
- Sample QC
- Library preparation and QC
- Advice on choice of sequencing platform
- Covaris E220 shearing of DNA/RNA samples; chromatin samples
- Close interaction with Bioinformatics support facility
Range of sequencing applications
- Transcriptome/RNA sequencing
- Chip-seq/ Cut&Run
- ATAC sequencing
- 10X single cell sequencing
- Small RNA sequencing
- Single cell transcriptome sequencing
- Whole Genome Bisulfite Sequencing
- Reduced Representation Bisulfite Sequencing
- Genomic resequencing libraries
- Amplicon sequencing
- Hi-C sequencing
- Specialist techniques (tRNA, riboseq, Chirp, MNase, RNA bisulfite seq, CRISPR, transposon mutagenesis screens, ultra low input samples, …)
- Individual projects and non-established methods will always be considered!
Sequencing
The sequencing itself is not done in-house, but we have a good working relationship with the CIGC here in Cambridge which allows us access to the latest Illumina sequencing technology. Currently we can offer a wide range of throughput needs on the Novaseq6000.
If you are using the NGS library facility, we will submit your libraries directly to the genome core at CIGC. If you are making your own libraries and would like access to the sequencing core, your group will need to set up an account with CIGC directly.