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Wellcome-MRC Cambridge Stem Cell Institute


Multi-user facility including superresolution confocal microscopy and live cell imaging.


Booking System



live cell


Zeiss LSM 710  30

Zeiss LSM 880 MP Airyscan

Zeiss LSM 700   30
Zeiss LSM 980 Airyscan2 30
Leica SP5 1   30
Leica SP5 2 30
Nikon spinning disk 30
CCMR SP8   30
Leica Stellaris 8 + FLIM 30
Zeiss Imager Apotome   14
Leica DMI600 Matrix HCS 14
Leica DMI4000   14
GE Incell 2200 Analyser   14
Essen Incucyte Zoom 40 plate/day

Nikon Biostation IM






Analysis PCs using remote desktop access

Imaris, autodeblur, Arivis Vision 4D -



Tescan Clara SEM, Katana serial microtome


Hitachi HT7800 Transmission Electron Microscope




Expert advice, assistance and training are available for the following:

  • All aspects of imaging for researchers
  • Image analysis and custom analysis tools
  • Processing of image volumes (deconvolution, 3D reconstruction)
  • Creation of figures for publication



The Imaging Facility is managed by Darran Clements 

For further information regarding services provided by the Cambridge Stem Cell Institute Imaging Core Facility, please contact: 

Booking System

If you need to request a booking system account, please click here.

Policy document


  • Stuart HT, Stirparo GG, Lohoff T, Bates LE, Kinoshita M, Lim CY, Sousa EJ, Maskalenka K, Radzisheuskaya A, Malcolm AA, Alves MRP, Lloyd RL, Nestorowa S, Humphreys P, Mansfield W, Reik W, Bertone P, Nichols J, Göttgens B, Silva JCR. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency. Cell Stem Cell. 2019 Sep 5;25(3):388-406.e8. doi: 10.1016/j.stem.2019.07.009. Epub 2019 Aug 15.
  • Alexandrova S, Kalkan T, Humphreys P, Roddell A, Scognamiglio R, Trumpp A, Nichols J. Selection and synamics of embryonic stem cell integration into early mouse embryos. Development  2016 Jan 1;143(1):24-34 doi: 10.1242/dev.124602
  • Driskell I, Oeztuerk-Winder F, Humphreys P, Frye M Genetically induced cell death in bulge stem cells reveals their redundancy for hair and epidermal regeneration. Stem Cells 2015 Mar;33(3):988-98 doi:10.1002/stem.1910
  • Blanco S, Dietmann S, Flores JV, Hussain S, Kutter C, Humphreys P, Lukk M, Lombard P, Treps L, Popis M, Kellner S, Hölter SM, Garrett L, Wurst W, Becker L, Klopstock T, Fuchs H, Gailus-Durner V, Hrabĕ de Angelis M, Káradóttir RT, Helm M, Ule J, Gleeson JG, Odom DT, Frye M. Aberrant methylation of tRNAs links cellular stress to neuro-developmental disorders EMBO J. 2014 Sep 17;33(18):2020-39. doi: 10.15252/embj.201489282. Epub 2014 Jul 25.
  • Leitch HG, Nichols J, Humphreys P, Mulas C, Martello G, Lee C, Jones K, Surani MA, Smith A. Rebuilding pluripotency from primordial germ cells Stem Cell Reports. 2013 Jun 4;1(1):66-78. doi: 10.1016/j.stemcr.2013.03.004. eCollection 2013.
  • Blair K, Leitch HG, Mansfield W, Dumeau CE, Humphreys P, Smith AG. Culture parameters for stable expansion, genetic modification and germline transmission of rat pluripotent stem cells Biology Open 2012 1:58-65; doi:10.1242/bio.2011029
  • Scherf N, Herberg M, Thierbach K, Zerjatke T, Kalkan T, Humphreys P, Smith A, Glauche I, Roeder I. Imaging, quantification and visualization of spatio-temporal patterning in mESC colonies under different culture conditions. Bioinformatics. 2012 Sep 15;28(18):i556-i561. doi: 10.1093/bioinformatics/bts404.
  • Reynolds N, Latos P, Hynes-Allen A, Loos R, Leaford D, O'Shaughnessy A, Mosaku O, Signolet J, Brennecke P, Kalkan T, Costello I, Humphreys P, Mansfield W, Nakagawa K, Strouboulis J, Behrens A, Bertone P, Hendrich B. NuRD suppresses pluripotency gene expression to promote transcriptional heterogeneity and lineage commitment. Cell Stem Cell. 2012 May 4;10(5):583-94.
  • Driskell I, Oda H, Blanco S, Nascimento E, Humphreys P, Frye M. The histone methyltransferase Setd8 acts in concert with c-Myc and is required to maintain skin. EMBO J. 2011 Nov 25. doi: 10.1038/emboj.2011.421. [Epub ahead of print] PubMed PMID: 22117221.
  • Ng K, Daigle N, Bancaud A, Ohhata T, Humphreys P, Walker R, Ellenberg J, Wutz A. A system for imaging the regulatory noncoding Xist RNA in living mouse embryonic stem cells. Mol Biol Cell. 2011 Jul 15;22(14):2634-45. Epub 2011 May 25. PubMed PMID: 21613549; PubMed Central PMCID: PMC3135487.
  • Guo G, Huang Y, Humphreys P, Wang X, Smith A. A PiggyBac-based recessive screening method to identify pluripotency regulators. PLoS One. 2011 Apr 18;6(4):e18189. PubMed PMID: 21533166; PubMed Central PMCID: PMC3078921. 
  • Danovi D, Falk A, Humphreys P, Vickers R, Tinsley J, Smith AG, Pollard SM. Imaging-based chemical screens using normal and glioma-derived neural stem cells. Biochem Soc Trans. 2010 Aug;38(4):1067-71. Review. PubMed PMID: 20659005. 
  • Leitch HG, Blair K, Mansfield W, Ayetey H, Humphreys P, Nichols J, Surani MA, Smith A. Embryonic germ cells from mice and rats exhibit properties consistent with a generic pluripotent ground state. Development. 2010 Jul;137(14):2279-87. Epub 2010 Jun 2. PubMed PMID: 20519324; PubMed Central PMCID: PMC2889601. 
  • Hall J, Guo G, Wray J, Eyres I, Nichols J, Grotewold L, Morfopoulou S, Humphreys P, Mansfield W, Walker R, Tomlinson S, Smith A. Oct4 and LIF/Stat3 additively induce Krüppel factors to sustain embryonic stem cell self-renewal. Cell Stem Cell. 2009 Dec 4;5(6):597-609. PubMed PMID: 19951688. 
  • Hussain S, Benavente SB, Nascimento E, Dragoni I, Kurowski A, Gillich A, Humphreys P, Frye M. The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability. J Cell Biol. 2009 Jul 13;186(1):27-40. PubMed PMID: 19596847; PubMed Central PMCID: PMC2712989.