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Wellcome-MRC Cambridge Stem Cell Institute


CSCI Tissue culture facility


The Tissue Culture Facility provides services primarily to the CSCI but some services are available to all university departments. Please email if you would like to us any of our services:

Aliquot productions

The Tissue Culture Facility provides sterile aliquots in single use/low volumes. These aliquots are available to buy by all labs in the University of Cambridge. We can also supply other universities with a selection of our products.



FBS tested for mES sutiablility - 5, 50, 500ml

Sabouraud broth to detect yeast -9ml 

EU approved general FBS - 5, ,20, 50, 500ml

Thioglycollate medium to detect bacteria - 5ml 

FBS tested for haematopoietic progenitors - 500ml

Soyabean broth to detect bacteria - 5ml 

KnockOutSerumReplacement serum - 50ml, 500ml

Mycoplasma broths


Lenti-X GoStix test - testing for lentivirsus

penicillin/streptomycin 100x - 5.5ml, 100ml


Blasticidin 10mg/ml - 200µl, 50µl

Gelatin 1 %  - 30ml  

4hydroxytamoxifen 1mM - 0.5ml 

Gelatin 0.1% - 550ml

puromycin solution (10mg/ml) - 1ml, 20ul

Trypan blue - 100ml

G418 [Geneticin] (50mg/ml) - 4ml, 1ml

TC grade water - 500ml 

hygromycin B 50 mg/ml - 1ml, 500ul

DMSO cell culture - 100ml

Zeocin 100mg/ml- 50, 500, 700ul

PBS Ca&Mg free - 500ml  Sigma

 Media additives

PBS Ca& Mg free - 500ml  Gibco

L-glutamine 200mM- 5.5ml, 100ml

BSA (0.1% ) in PBS - 500ml 

MEM non essential amino acids - 5.5ml, 100ml


Sodium pyruvate - 5.5ml, 100ml

TAT cre 0.5mM - 50µl

Insulin Human 5ml @10mg/ml- 275ul, 5ml

TAT dre 0.2mM - 100µl

Bovine Insulin 5mg/ml - 500µl

Human BMP2  - 250ug

Mouse Lif 10µg/ml - 550µl 

Ms FGF4 - 400ug

Human Lif 10µg/ml - 550µl 

Activin A - 250ug

Activin A2 0.5mg/ml - 10µl  

Human BDNF - 100ug

FGF2 50µg/ml - 100µl, 50µl 

eSpCas9 5.3 mg/ml ( 32 µM) - 50ul

Noggin 100ug/ml - 40ul

Cas9  4.8mg/ml - 50ul

FGF10 100ug/ml - 40ul

50ug recombinant human FGF10

FGF7 50ug/ml - 80ul

25ug recombinant human TGFβ1 PLUS

EGF 500ug/ml - 20ul

50ug recombinant human HGF

Chiron 10mM - 15, 30, 45 60µl

50ug recombinant human Gremlin 1

PD03 10mM- 15, 30, 45 60µl

50ug recombinant human GDF15

 Made to order/batch tested reagents

25ug recombinant mouse Noggin

 HCE - 100ml aliquoted to order

25ug recombinant human BMP4

Adenine 4.86mg/ml- 400x 2.5ml

25ug recombinant human FGF-9

Apotransferrin 5mg/ml - 384x510ul

50ug recombinant human FGF-4 protein

L-Ascorbic acid (Vit C) 250mM 135x 500µl

100ug recombinant human/bovine/porcine IGF-1 LR3 protein

RSPO1 Conditioned media - order in advance

25ug recombinant human PDGF-AA protein

WNT3a Conditioned media - order in advance

25ug recombinant human PDGF-BB protein

Homemade N2 - 5ml

25ug recombinant human KGF (FGF-7) protein

Homemade BN2 - 56 ml, 112ml aliquoted to order

 Detachment reagents

Neurobasal Media - 500ml Batch tested

TRYPSIN EDTA 0.25% - 17ml 

B27 - 10ml batch tested, 17x530µl

Accutase - 17ml, 100ml


Triple express - 500 ml 

For an up-to-date copy of our order form with sizes and prices please email:


Mycoplasma Screening – JCBC only

Mycoplasma infections of cells in culture were first discovered in 1956 and 10 years later it was reported that 59% of cells in culture were infected with mycoplasma. Mycoplasma infections may induce cellular changes, including chromosome aberrations, changes in metabolism and cell growth.

The Tissue Culture Facility routinely run a PCR-based mycoplasma test which is available to labs in the JCBC. These tests are sensitive down to 500CFU/ml. To submit a sample for testing all we need is a sample of media from your cells that is antibiotic free and has been on the cells for 5 days.


Plasticware – JCBC only

The TC team bulk purchase plastic consumables for the TC labs, ensuring the best price. These are delivered to the users normally with same day delivery. CSCI members can find the plasticware order form on the facilities shared drive.

Link for PC users – if you are offsite you need to be connected to the network via VPN


Link for Mac users – if you are offsite you need to be connected to the network via VPN




The Academic chair for Tissue Culture is Dr Maria Alcolea.

For general enquiries about the services that the Tissue Culture team provide please email on: or call on: 01223 760293