CSCI Tissue culture facility
The Tissue Culture Facility provides services primarily to CSCI, but some services are available to all university departments. Please email if you would like to use any of our services: tissue-culture@stemcells.cam.ac.uk
Aliquot productions
The Tissue Culture Facility provides sterile aliquots in single use/low volumes. These aliquots are available to buy for all academic labs.
Antibiotics |
Broths/tests |
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penicillin/streptomycin 100x - 5.5ml, 100ml |
Sabouraud broth to detect yeast -9ml |
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Blasticidin 10mg/ml - 50µl |
Thioglycolate medium to detect bacteria - 5ml |
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4hydroxytamoxifen 1mM - 0.5ml |
Soyabean broth to detect bacteria - 5ml |
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puromycin solution (10mg/ml) - 1ml, 20ul |
Mycoplasma strip tests |
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G418 [Geneticin] (50mg/ml) - 4ml, 1ml |
Lenti-X GoStix test - testing for lentivirus |
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hygromycin B 50 mg/ml - 1ml |
Misc |
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Zeocin 100mg/ml- 1.25ml |
Gelatin 1 % - 30ml |
Media additives |
Gelatin 0.1% - 550ml |
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L-glutamine 200mM- 5.5ml, 100ml |
Trypan blue - 100ml |
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MEM non-essential amino acids - 5.5ml, 100ml |
TC grade water - 500ml |
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Sodium pyruvate - 5.5ml, 100ml |
DMSO cell culture - 100ml |
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Insulin Human 5ml @10mg/ml- 275ul, 5ml |
PBS Ca&Mg free - 500ml Sigma |
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Bovine Insulin 5mg/ml - 500µl |
PBS Ca& Mg free - 500ml Gibco |
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Mouse Lif 10µg/ml - 550µl |
BSA (0.1%) in PBS - 500ml |
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Human Lif 10µg/ml - 550µl |
Biochem Proteins |
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Activin A2 0.5mg/ml - 10µl |
TAT cre 0.5mM - 50µl |
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FGF2 50µg/ml - 100µl |
Activin A - 250ug |
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Noggin 100ug/ml - 40ul |
Human BDNF - 100ug |
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FGF10 100ug/ml - 40ul |
eSpCas9 5.3 mg/ml (32 µM) - 50ul |
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FGF7 50ug/ml - 80ul |
Cas9 4.8mg/ml - 50ul |
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Chiron 10mM - 15, 30, 45 60µl |
Detachment reagents |
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PD03 10mM- 15, 30, 45 60µl |
TRYPSIN EDTA 0.25% - 17ml |
Made to order/batch tested reagents |
Accutase - 17ml, 100ml |
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HCE - 100ml aliquoted to order |
Triple express - 500 ml |
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Adenine 4.86mg/ml- 400x 2.5ml |
Serum |
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Apotransferrin 5mg/ml - 384x510ul |
FBS evaluated for mES suitability - 5, 50, 500ml |
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RSPO1 Conditioned media - order in advance |
EU approved general FBS - 5, ,20, 50, 500ml |
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WNT3a Conditioned media - order in advance |
FBS evaluated for haematopoietic progenitors - 500ml |
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Homemade N2 - 5ml |
Knockout Serum Replacement serum - 50ml, 500ml |
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Homemade BN2 - 56 ml, 112ml aliquoted to order |
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Neurobasal Media - 500ml Batch tested |
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B27 - 10ml batch tested, 17x530µl |
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We also hold and supply Qkine proteins - https://qkine.com/
CSCI members can find the order forms in the department SharePoint
Non-employees should email enquiries to: tissue-culture@stemcells.cam.ac.uk
Mycoplasma Screening – JCBC only
The Tissue Culture Facility routinely run a PCR-based mycoplasma test which is available to labs in the JCBC for a cost of £3/sample or £60 for an urgent test of up to 20 samples. The PCR tests are sensitive down to 500CFU/ml. To submit a sample for testing all we need is a sample of media from your cells that is antibiotic free and has been on the cells for 5 days.
We can also run rapid tests of up to 8 samples using the Invivogen mycostrips, but this test is less sensitive and detects fewer species of mycoplasma.
Order forms and details of how to prepare samples can be found on SharePoint.
Plasticware – Biomedical Campus only
The TC team bulk purchase plastic consumables for the TC labs, ensuring the best price. These are delivered to the users normally with same day delivery. CSCI members can find the plasticware order forms on SharePoint.
Non CSCI members should email the TC team for the latest version of the order form.
Contact
The Academic chair for Tissue Culture is Maria Alcolea.
For general enquiries about the services provided by the Tissue Culture team, please email: tissue-culture@stemcells.cam.ac.uk or call: 01223 760293