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Wellcome - MRC

Cambridge Stem Cell Institute

Studying at Cambridge

 

Imaging

Multi-user facility including confocal microscopy, live cell imaging, high content screening and colony analysis

 

Equipment

An extensively used multiuser facility comprising of:

  • Zeiss 710 FLIM
  • Zeiss 880 MP
  • 2x Leica SP5 TCS confocal microscopes
  • Andor Revolution XD Spinning Disk microscope
  • Leica Matrix HCS screening / live cell imaging microscope
  • Zeiss Imager H&E and fluorescence microscope
  • Flourescent inverted microscopes for Tissue culture
  • Incucyte time lapse systems
  • Nikon Biostation IM time lapse system

Expertise

Expert advice, assistance and training are available for the following:

  • All aspects of imaging for researchers
  • Image analysis and custom analysis tools
  • Processing of image volumes (deconvolution, 3D reconstruction)
  • Creation of figures for publication

 

Imaging facility example

 

Contact

The Imaging Facility is managed by .

For further information regarding services provided by the Cambridge Stem Cell Institute Imaging Core Facility, please contact: 

To make a booking, please go to the online Booking System.

If you need to request a booking system account, please click here.

 

Publications

  • Alexandrova S, Kalkan T, Humphreys P, Roddell A, Scognamiglio R, Trumpp A, Nichols J. Selection and synamics of embryonic stem cell integration into early mouse embryos. Development  2016 Jan 1;143(1):24-34 doi: 10.1242/dev.124602
  • Driskell I, Oeztuerk-Winder F, Humphreys P, Frye M Genetically induced cell death in bulge stem cells reveals their redundancy for hair and epidermal regeneration. Stem Cells 2015 Mar;33(3):988-98 doi:10.1002/stem.1910
  • Blanco S, Dietmann S, Flores JV, Hussain S, Kutter C, Humphreys P, Lukk M, Lombard P, Treps L, Popis M, Kellner S, Hölter SM, Garrett L, Wurst W, Becker L, Klopstock T, Fuchs H, Gailus-Durner V, Hrabĕ de Angelis M, Káradóttir RT, Helm M, Ule J, Gleeson JG, Odom DT, Frye M. Aberrant methylation of tRNAs links cellular stress to neuro-developmental disorders EMBO J. 2014 Sep 17;33(18):2020-39. doi: 10.15252/embj.201489282. Epub 2014 Jul 25.
  • Leitch HG, Nichols J, Humphreys P, Mulas C, Martello G, Lee C, Jones K, Surani MA, Smith A. Rebuilding pluripotency from primordial germ cells Stem Cell Reports. 2013 Jun 4;1(1):66-78. doi: 10.1016/j.stemcr.2013.03.004. eCollection 2013.
  • Blair K, Leitch HG, Mansfield W, Dumeau CE, Humphreys P, Smith AG. Culture parameters for stable expansion, genetic modification and germline transmission of rat pluripotent stem cells Biology Open 2012 1:58-65; doi:10.1242/bio.2011029
  • Scherf N, Herberg M, Thierbach K, Zerjatke T, Kalkan T, Humphreys P, Smith A, Glauche I, Roeder I. Imaging, quantification and visualization of spatio-temporal patterning in mESC colonies under different culture conditions. Bioinformatics. 2012 Sep 15;28(18):i556-i561. doi: 10.1093/bioinformatics/bts404.
  • Reynolds N, Latos P, Hynes-Allen A, Loos R, Leaford D, O'Shaughnessy A, Mosaku O, Signolet J, Brennecke P, Kalkan T, Costello I, Humphreys P, Mansfield W, Nakagawa K, Strouboulis J, Behrens A, Bertone P, Hendrich B. NuRD suppresses pluripotency gene expression to promote transcriptional heterogeneity and lineage commitment. Cell Stem Cell. 2012 May 4;10(5):583-94.
  • Driskell I, Oda H, Blanco S, Nascimento E, Humphreys P, Frye M. The histone methyltransferase Setd8 acts in concert with c-Myc and is required to maintain skin. EMBO J. 2011 Nov 25. doi: 10.1038/emboj.2011.421. [Epub ahead of print] PubMed PMID: 22117221.
  • Ng K, Daigle N, Bancaud A, Ohhata T, Humphreys P, Walker R, Ellenberg J, Wutz A. A system for imaging the regulatory noncoding Xist RNA in living mouse embryonic stem cells. Mol Biol Cell. 2011 Jul 15;22(14):2634-45. Epub 2011 May 25. PubMed PMID: 21613549; PubMed Central PMCID: PMC3135487.
  • Guo G, Huang Y, Humphreys P, Wang X, Smith A. A PiggyBac-based recessive screening method to identify pluripotency regulators. PLoS One. 2011 Apr 18;6(4):e18189. PubMed PMID: 21533166; PubMed Central PMCID: PMC3078921. 
  • Danovi D, Falk A, Humphreys P, Vickers R, Tinsley J, Smith AG, Pollard SM. Imaging-based chemical screens using normal and glioma-derived neural stem cells. Biochem Soc Trans. 2010 Aug;38(4):1067-71. Review. PubMed PMID: 20659005. 
  • Leitch HG, Blair K, Mansfield W, Ayetey H, Humphreys P, Nichols J, Surani MA, Smith A. Embryonic germ cells from mice and rats exhibit properties consistent with a generic pluripotent ground state. Development. 2010 Jul;137(14):2279-87. Epub 2010 Jun 2. PubMed PMID: 20519324; PubMed Central PMCID: PMC2889601. 
  • Hall J, Guo G, Wray J, Eyres I, Nichols J, Grotewold L, Morfopoulou S, Humphreys P, Mansfield W, Walker R, Tomlinson S, Smith A. Oct4 and LIF/Stat3 additively induce Krüppel factors to sustain embryonic stem cell self-renewal. Cell Stem Cell. 2009 Dec 4;5(6):597-609. PubMed PMID: 19951688. 
  • Hussain S, Benavente SB, Nascimento E, Dragoni I, Kurowski A, Gillich A, Humphreys P, Frye M. The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability. J Cell Biol. 2009 Jul 13;186(1):27-40. PubMed PMID: 19596847; PubMed Central PMCID: PMC2712989.