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Wellcome - MRC Cambridge Stem Cell Institute

 

The electron microscopy core facility offers a range of specimen preparation services and training for electron microscopy approaches for academic users across Cambridge and externally. The facility focusses on biomedical applications and works with a huge range of specimens from isolated viruses to tissue samples.

Please contact Scott Dillon () with queries. New and experienced electron microscopists alike are encouraged to get in touch with us to discuss their projects and how the facility can help. We are very happy to discuss projects at an early stage of development and undertake method development on a collaborative basis.

All of our services and equipment are accessed through the PPMS booking system.


Sample preparation services

  • Routine and tailored staining and resin embedding for biological samples.
  • Semithin and ultrathin sectioning.
  • Heavy metal staining.
  • Negative staining.

Coming soon:

  • Immuno-electron microscopy (Tokuyasu sectioning and immungold staining).
  • Pre-embedding immunogold staining.
  • Preparation for correlative light-electron microscopy.

Microscopes

Hitachi HT7800 TEM

The HT7800 is an 80-120keV transmission electron microscope with a LaB6 filament which is designed for user-friendly imaging.

  • LaB6 electron source operated at accelerating voltages of between 80-120keV.
  • EMSIS Xarosa 20 megapixel CMOS camera allowing 5,120 x 3,840px images to be acquired.
  • Integrated screen camera allowing operation under normal room light and easy sample navigation.
  • Lattice resolution of 0.204 nm (off-axis, 100keV).
  • Software functions for image montaging, intuitive sample navigation and automated image acquisition.
  • CompuStage with ± 70° stage tilt for electron tomography.

Cost: £30/h internal; £53/h external/assisted. Please enquire for industry rates.

The TEM was funded by a Wellcome Trust multi-user equipment grant (212912/Z/18/Z) and additional generous support from the Cambridge Stem Cell Institute, MRC Mitochondrial Biology Unit, UK Dementia Research Institute and the Issac Newton Trust.

We are currently in the process of purchasing a scanning electron microscope to expand our capabilities - watch this space!


Preparation equipment

Leica EM UC7 ultramicrotome

Our Leica EM UC7 ultramicrotome is fully equipped for semithin and ultrathin sectioning for biological samples and is also enabled for low temperature sectioning.

  • Semithin and ultrathin sectioning of resin samples.
  • Integrated EM FC7 cryochamber with CRION ioniser for low temperature sectioning allowing immunoEM approaches.