CSCI Tissue culture facility
The Tissue Culture Facility provides services primarily to the CSCI but some services are available to all university departments. Please email if you would like to us any of our services: tissue-culture@stemcells.cam.ac.uk
Aliquot productions
The Tissue Culture Facility provides sterile aliquots in single use/low volumes. These aliquots are available to buy by all labs in the University of Cambridge. We can also supply other universities with a selection of our products.
Serum |
Broths/tests |
FBS tested for mES sutiablility - 5, 50, 500ml |
Sabouraud broth to detect yeast -9ml |
EU approved general FBS - 5, ,20, 50, 500ml |
Thioglycollate medium to detect bacteria - 5ml |
FBS tested for haematopoietic progenitors - 500ml |
Soyabean broth to detect bacteria - 5ml |
KnockOutSerumReplacement serum - 50ml, 500ml |
Mycoplasma broths |
Antibiotics |
Lenti-X GoStix test - testing for lentivirsus |
penicillin/streptomycin 100x - 5.5ml, 100ml |
Misc |
Blasticidin 10mg/ml - 200µl, 50µl |
Gelatin 1 % - 30ml |
4hydroxytamoxifen 1mM - 0.5ml |
Gelatin 0.1% - 550ml |
puromycin solution (10mg/ml) - 1ml, 20ul |
Trypan blue - 100ml |
G418 [Geneticin] (50mg/ml) - 4ml, 1ml |
TC grade water - 500ml |
hygromycin B 50 mg/ml - 1ml, 500ul |
DMSO cell culture - 100ml |
Zeocin 100mg/ml- 50, 500, 700ul |
PBS Ca&Mg free - 500ml Sigma |
Media additives |
PBS Ca& Mg free - 500ml Gibco |
L-glutamine 200mM- 5.5ml, 100ml |
BSA (0.1% ) in PBS - 500ml |
MEM non essential amino acids - 5.5ml, 100ml |
Proteins |
Sodium pyruvate - 5.5ml, 100ml |
TAT cre 0.5mM - 50µl |
Insulin Human 5ml @10mg/ml- 275ul, 5ml |
TAT dre 0.2mM - 100µl |
Bovine Insulin 5mg/ml - 500µl |
Human BMP2 - 250ug |
Mouse Lif 10µg/ml - 550µl |
Ms FGF4 - 400ug |
Human Lif 10µg/ml - 550µl |
Activin A - 250ug |
Activin A2 0.5mg/ml - 10µl |
Human BDNF - 100ug |
FGF2 50µg/ml - 100µl, 50µl |
eSpCas9 5.3 mg/ml ( 32 µM) - 50ul |
Noggin 100ug/ml - 40ul |
Cas9 4.8mg/ml - 50ul |
FGF10 100ug/ml - 40ul |
50ug recombinant human FGF10 |
FGF7 50ug/ml - 80ul |
25ug recombinant human TGFβ1 PLUS |
EGF 500ug/ml - 20ul |
50ug recombinant human HGF |
Chiron 10mM - 15, 30, 45 60µl |
50ug recombinant human Gremlin 1 |
PD03 10mM- 15, 30, 45 60µl |
50ug recombinant human GDF15 |
Made to order/batch tested reagents |
25ug recombinant mouse Noggin |
HCE - 100ml aliquoted to order |
25ug recombinant human BMP4 |
Adenine 4.86mg/ml- 400x 2.5ml |
25ug recombinant human FGF-9 |
Apotransferrin 5mg/ml - 384x510ul |
50ug recombinant human FGF-4 protein |
L-Ascorbic acid (Vit C) 250mM 135x 500µl |
100ug recombinant human/bovine/porcine IGF-1 LR3 protein |
RSPO1 Conditioned media - order in advance |
25ug recombinant human PDGF-AA protein |
WNT3a Conditioned media - order in advance |
25ug recombinant human PDGF-BB protein |
Homemade N2 - 5ml |
25ug recombinant human KGF (FGF-7) protein |
Homemade BN2 - 56 ml, 112ml aliquoted to order |
Detachment reagents |
Neurobasal Media - 500ml Batch tested |
TRYPSIN EDTA 0.25% - 17ml |
B27 - 10ml batch tested, 17x530µl |
Accutase - 17ml, 100ml |
Triple express - 500 ml |
For an up-to-date copy of our order form with sizes and prices please email: tissue-culture@stemcells.cam.ac.uk
Mycoplasma Screening – JCBC only
Mycoplasma infections of cells in culture were first discovered in 1956 and 10 years later it was reported that 59% of cells in culture were infected with mycoplasma. Mycoplasma infections may induce cellular changes, including chromosome aberrations, changes in metabolism and cell growth.
The Tissue Culture Facility routinely run a PCR-based mycoplasma test which is available to labs in the JCBC. These tests are sensitive down to 500CFU/ml. To submit a sample for testing all we need is a sample of media from your cells that is antibiotic free and has been on the cells for 5 days.
Plasticware – JCBC only
The TC team bulk purchase plastic consumables for the TC labs, ensuring the best price. These are delivered to the users normally with same day delivery. CSCI members can find the plasticware order form on the facilities shared drive.
Link for PC users – if you are offsite you need to be connected to the network via VPN
\\jcbc-store01.jcbc.private.cam.ac.uk\csci_grp_facilities\facility_shares\tissue_culture
Link for Mac users – if you are offsite you need to be connected to the network via VPN
smb://jcbc-store01.jcbc.private.cam.ac.uk/csci_grp_facilities/facility_shares/tissue_culture
Contact
The Academic chair for Tissue Culture is Dr Maria Alcolea.
For general enquiries about the services that the Tissue Culture team provide please email on: tissue-culture@stemcells.cam.ac.uk or call on: 01223 760293